The daily virus sampling tube is used to collect influenza virus, hand, foot and mouth virus, measles and rubella virus and related samples. Short term storage and transportation at 2-8 ° C and long-term storage in refrigerator or liquid nitrogen environment at - 80 ° C. Specific uses of virus sampling tube:
1. Virus sampling tubes are generally used for nucleic acid extraction and later virus isolation of influenza virus, hand, foot and mouth virus, rubella virus and other samples from clinical patients. The amount of liquid required is usually 35m or 5m( (to be used with nucleic acid detection kit and spore culture medium) 2. The virus sampling tube can be used for the collection and short-term transport of avian influenza virus in the external environment. The amount of liquid required is usually 6ml. 3. The virus sampling tube can be used for daily monitoring and sampling of poultry, pigs and other animals. The amount of liquid required is usually 15m. 4. Virus sampling tube can be used for short-term preservation of virus samples( Store at 2-8 ° C for 48 hours) for necessary cell culture. 5. Virus sampling tube can be used for long-term preservation of virus samples (- 70 ℃ - 196 ℃ for one year) for necessary cell culture. 6. Virus sampling tube can be used to collect clinical respiratory virus rapid detection kit. 7. The virus sampling tube can be used to collect clinical samples of mycoplasma, chlamydia and Ureaplasma. 8. The virus sampling tube can be used to transport tissue samples from specific sites to the detection laboratory for necessary cell culture. Sampling method for virus collection by virus sampling tube: 1. First press the tongue with the tongue depressor, then extend the sampling swab head into the throat and wipe the bilateral pharyngeal tonsils and the posterior wall of the pharynx. Gently wipe the back wall of the pharynx to avoid touching the tongue. 2. Measure the distance from the tip of the nose to the earlobe with a sampling Cotton Swab and mark it with your fingers. Insert the sampling swab into the nasal cavity in a direction perpendicular to the nose (face). Stay in the nose for 15-30 seconds, rotate gently 3-5 times, and take out the swab.
It is not difficult to see from the method of use that the sampling of both oropharyngeal swabs and nasopharyngeal swabs is technical, difficult and easy to pollute. The quality of the collected samples is directly related to the subsequent detection. If the viral load of the collected samples is low, it is easy to cause false negative and difficult to diagnose. Some sampling swabs have low viral load, inappropriate breakpoints and are not easy for operators to break, which not only limits the sampling efficiency, but also easily leads to false negative nucleic acid detection. Therefore, the sampling swab head should be flocked with synthetic fiber instead of cotton or calcium alginate sponge. For the material of cotton swab handle, it is recommended to use hollow polystyrene (PS) extruded tube or polypropylene (PP) injection molded indentation tube instead of wooden stick or bamboo stick. Because the wooden stick or bamboo stick containing calcium alginate and wood components is damaged and soaked in the preservation solution, it will adsorb protein and even interfere with the subsequent detection. Cotton fiber has strong adsorption on protein and is not easy to wash off. The length of the swab shall not be too short, and the fracture position shall not be too close to the swab head, so as not to pollute the patient's body fluid during operation; The location of the breaking point shall be reasonable. When the virus sampling tube is broken, the risk of pollution caused by liquid splashing in the tube shall be avoided.
